Generator

Part:BBa_K1073014:Design

Designed by: iGEM Team Braunschweig 2013   Group: iGEM13_Braunschweig   (2013-09-16)

Constitutively expressed transcription regulator RhlR + LVA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 302
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 777


Design Notes

no considerations.


Source

This part is a combination of the BBa_J23100, BBa_B0032 and BBa_C0071, which were taken from the 2013 distribution kit.

References

[Medina et al., 2003] Medina G., et al., Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J Bacteriol. 2003 Oct; 185(20): 5976-83. PMID: 14526008